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rabbit polyclonal anti id1 antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rabbit polyclonal anti id1 antibody
    Rabbit Polyclonal Anti Id1 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 462 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-id1+polyclonal+antibody/Id1+Antibody/pm32767771-75-4-8
    Average 95 stars, based on 462 article reviews
    rabbit polyclonal anti id1 antibody - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: <p>Inhibitor of DNA binding 1 (Id1) mediates stemness of colorectal cancer cells through the Id1-c-Myc-PLAC8 axis via the Wnt/β-catenin and Shh signaling pathways</p>
    Article Snippet: After immunoblotting, membranes were blocked in 3% bovine serum albumin (BSA; VWR International, Radnor, PA, USA) at room temperature for 2 h. Then, the membrane was incubated with primary mouse anti-CD24 monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), mouse anti-CD133-2 monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), rabbit antiMUC2 monoclonal antibody (1:5000; Abcam, Inc.; Cambridge,MA, USA), rabbit anti-Lgr5monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), rabbit anti-Id1 polyclonal antibody (1:200; Santa Cruz Biotechnology; Santa Cruz, CA, USA), rabbit β-catenin monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), rabbit antiTwist, -Snail, -Slug, -twist, EpCAM, CD166, CyclinD1 (Santa Cruz Biotechnology; Santa Cruz, CA, USA), CD44, EZH2, snail, slug, OCT4 (Cell Signaling Technology; Beverly, MA, USA) and β-actin (Santa Cruz Biotechnology; Santa Cruz, CA, USA), respectively overnight at 4 °C.

    Membrane:

    Article Title: <p>Inhibitor of DNA binding 1 (Id1) mediates stemness of colorectal cancer cells through the Id1-c-Myc-PLAC8 axis via the Wnt/β-catenin and Shh signaling pathways</p>
    Article Snippet: After immunoblotting, membranes were blocked in 3% bovine serum albumin (BSA; VWR International, Radnor, PA, USA) at room temperature for 2 h. Then, the membrane was incubated with primary mouse anti-CD24 monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), mouse anti-CD133-2 monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), rabbit antiMUC2 monoclonal antibody (1:5000; Abcam, Inc.; Cambridge,MA, USA), rabbit anti-Lgr5monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), rabbit anti-Id1 polyclonal antibody (1:200; Santa Cruz Biotechnology; Santa Cruz, CA, USA), rabbit β-catenin monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), rabbit antiTwist, -Snail, -Slug, -twist, EpCAM, CD166, CyclinD1 (Santa Cruz Biotechnology; Santa Cruz, CA, USA), CD44, EZH2, snail, slug, OCT4 (Cell Signaling Technology; Beverly, MA, USA) and β-actin (Santa Cruz Biotechnology; Santa Cruz, CA, USA), respectively overnight at 4 °C.

    Incubation:

    Article Title: <p>Inhibitor of DNA binding 1 (Id1) mediates stemness of colorectal cancer cells through the Id1-c-Myc-PLAC8 axis via the Wnt/β-catenin and Shh signaling pathways</p>
    Article Snippet: After immunoblotting, membranes were blocked in 3% bovine serum albumin (BSA; VWR International, Radnor, PA, USA) at room temperature for 2 h. Then, the membrane was incubated with primary mouse anti-CD24 monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), mouse anti-CD133-2 monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), rabbit antiMUC2 monoclonal antibody (1:5000; Abcam, Inc.; Cambridge,MA, USA), rabbit anti-Lgr5monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), rabbit anti-Id1 polyclonal antibody (1:200; Santa Cruz Biotechnology; Santa Cruz, CA, USA), rabbit β-catenin monoclonal antibody (1:5000; Abcam, Inc.; Cambridge, MA, USA), rabbit antiTwist, -Snail, -Slug, -twist, EpCAM, CD166, CyclinD1 (Santa Cruz Biotechnology; Santa Cruz, CA, USA), CD44, EZH2, snail, slug, OCT4 (Cell Signaling Technology; Beverly, MA, USA) and β-actin (Santa Cruz Biotechnology; Santa Cruz, CA, USA), respectively overnight at 4 °C.



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    Santa Cruz Biotechnology rabbit anti id1 polyclonal antibody
    HEMF secreted factors modulate BMP signaling in squamous epithelial cells. ( a ) pSMAD1/5/8 immunofluorescent staining (Cy2, green) was performed on paraffin-embedded, formalin-fixed sections of 3D organotypic ALI culture established with control serum-free myofibroblast media (SFMM), conditioned SFMM (cSFMM), and cSFMM plus the addition of GREM1 nAb. Representative images of three independent experiments is shown. Images were viewed with a confocal microscope. Magnification 40x. ( b ) Immunoblots for pSMAD1/5/8 and <t>ID1</t> were performed on epithelium harvested from 3D organotypic ALI culture. Epithelial pSMAD1/5/8 and ID1 increase in the presence of cSFMM. Addition of GREM1 nAb inhibits the increase in pSMAD1/5/8 and ID1. Tubulin was used as the loading control. Representative immunoblots are shown of three individual experiments. Samples from each experiment were derived at the same time and processed in parallel. Relative densitometry to loading control tubulin is shown. *P ≤ 0.001; **P ≤ 0.02; **P ≤ 0.05; NS, non-significant with ANOVA for multiple comparisons.
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    Santa Cruz Biotechnology rabbit polyclonal anti id1 z 8 antibody
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    Santa Cruz Biotechnology rabbit polyclonal antibody against human id 1
    HEMF secreted factors modulate BMP signaling in squamous epithelial cells. ( a ) pSMAD1/5/8 immunofluorescent staining (Cy2, green) was performed on paraffin-embedded, formalin-fixed sections of 3D organotypic ALI culture established with control serum-free myofibroblast media (SFMM), conditioned SFMM (cSFMM), and cSFMM plus the addition of GREM1 nAb. Representative images of three independent experiments is shown. Images were viewed with a confocal microscope. Magnification 40x. ( b ) Immunoblots for pSMAD1/5/8 and <t>ID1</t> were performed on epithelium harvested from 3D organotypic ALI culture. Epithelial pSMAD1/5/8 and ID1 increase in the presence of cSFMM. Addition of GREM1 nAb inhibits the increase in pSMAD1/5/8 and ID1. Tubulin was used as the loading control. Representative immunoblots are shown of three individual experiments. Samples from each experiment were derived at the same time and processed in parallel. Relative densitometry to loading control tubulin is shown. *P ≤ 0.001; **P ≤ 0.02; **P ≤ 0.05; NS, non-significant with ANOVA for multiple comparisons.
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    Image Search Results


    HEMF secreted factors modulate BMP signaling in squamous epithelial cells. ( a ) pSMAD1/5/8 immunofluorescent staining (Cy2, green) was performed on paraffin-embedded, formalin-fixed sections of 3D organotypic ALI culture established with control serum-free myofibroblast media (SFMM), conditioned SFMM (cSFMM), and cSFMM plus the addition of GREM1 nAb. Representative images of three independent experiments is shown. Images were viewed with a confocal microscope. Magnification 40x. ( b ) Immunoblots for pSMAD1/5/8 and ID1 were performed on epithelium harvested from 3D organotypic ALI culture. Epithelial pSMAD1/5/8 and ID1 increase in the presence of cSFMM. Addition of GREM1 nAb inhibits the increase in pSMAD1/5/8 and ID1. Tubulin was used as the loading control. Representative immunoblots are shown of three individual experiments. Samples from each experiment were derived at the same time and processed in parallel. Relative densitometry to loading control tubulin is shown. *P ≤ 0.001; **P ≤ 0.02; **P ≤ 0.05; NS, non-significant with ANOVA for multiple comparisons.

    Journal: Scientific Reports

    Article Title: Human esophageal myofibroblast secretion of bone morphogenetic proteins and GREMLIN1 and paracrine regulation of squamous epithelial growth

    doi: 10.1038/s41598-018-30799-7

    Figure Lengend Snippet: HEMF secreted factors modulate BMP signaling in squamous epithelial cells. ( a ) pSMAD1/5/8 immunofluorescent staining (Cy2, green) was performed on paraffin-embedded, formalin-fixed sections of 3D organotypic ALI culture established with control serum-free myofibroblast media (SFMM), conditioned SFMM (cSFMM), and cSFMM plus the addition of GREM1 nAb. Representative images of three independent experiments is shown. Images were viewed with a confocal microscope. Magnification 40x. ( b ) Immunoblots for pSMAD1/5/8 and ID1 were performed on epithelium harvested from 3D organotypic ALI culture. Epithelial pSMAD1/5/8 and ID1 increase in the presence of cSFMM. Addition of GREM1 nAb inhibits the increase in pSMAD1/5/8 and ID1. Tubulin was used as the loading control. Representative immunoblots are shown of three individual experiments. Samples from each experiment were derived at the same time and processed in parallel. Relative densitometry to loading control tubulin is shown. *P ≤ 0.001; **P ≤ 0.02; **P ≤ 0.05; NS, non-significant with ANOVA for multiple comparisons.

    Article Snippet: The membrane was blocked with 5% milk or BSA and incubated overnight with rabbit anti-p63 (0.33 μg/ml, Genetex, #GTX102425), mouse anti-PCNA (1:400, BD Transduction, cat#610664), rabbit anti-pSMAD1/5/8 monoclonal (1:500, Cell Signaling, cat# 9516), rabbit anti-SMAD5 monoclonal (1:1000, Cell Signaling, cat# 12534 S), or rabbit anti-ID1 polyclonal antibody (0.5 μg/ml, Santa Cruz #sc-488).

    Techniques: Staining, Control, Microscopy, Western Blot, Derivative Assay